When the mysterious Espella is pursued through London by a bewildering force, she desperately seeks help from legendary puzzle investigator Professor Layton and his assistant Luke Triton. However, before the duo are able to work out whats going on, Espella is suddenly kidnapped. While attempting to track her down, Layton and Luke encounter a strange book and are transported to the outlandish medieval city of Labyrinthia - a place allegedly inhabited by witches and magic!